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Introduction
Histology is the
microscopic study of tissues and cells with its
techniques producing visual images that are a real mix
of art and science. The Histology Laboratory is a
service facility in the Stem Cell Unit (SCU), Department
of Anatomy, Located on Level 1 in the College of
Medicine, King Saud University.
History
The Histology Laboratory
has a long history of providing technical services which
have been an essential part of the Department's
obligation to quality in teaching and research. In the
last two years major renovations took place resulting in
a well-equipped, state of the art Histology Facility,
compliant with standard requirements of safety.
Services*
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Tissue processing and
paraffin embedding.
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Sectioning of paraffin blocks.
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Cryostat sectioning of tissues.
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Hematoxylin and Eosin (H&E) staining.
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Special stains
including Masson’s trichrome for collagen, Alcian
Blue for sulfated mucosubstances, Verhoeff's-Van
Gieson and Orcein-Giemsa for elastic fibers, Carbol
chromotrope for eosinopils, oil red for fat, von
Kossa and alizarin red for calcium and other stains
as requested.
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Immunohistochemistry, immunocytochemistry and
immunofluorescence.
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High quality digital
photography and virtual microscopy.
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Image analysis**.
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Training in
histological techniques.
*These histological
services are available by prior arrangement only and
subject to availability of resources.
**Our analysis services are only for research purposes
and should not be used for clinical diagnosis.
Equipments
For paraffin-based
histology, the laboratory is equipped with an automated
tissue processing station, an embedding center, 2 rotary
microtomes and a safety cabinet (for manual processing
and staining); and for frozen sectioning, 1 cryostat.
The laboratory is also equipped with different kinds of
microscopes and is proud to have the world leading
Aperio ScanScope for virtual microscopy and image
analysis.
Policy and Procedure
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A
Work Request Form should be filled out.
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Specimens for paraffin processing should be
submitted to our Lab in 10% formalin (preferably
buffered), or other fixative, in plastic containers
that seal. It is a good idea to consult us before
bringing the samples.
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Each specimen will be given an accession number
according to our coding system and will be
registered electronically to follow its progression.
Staff
They work with the same
team spirit that prevails in the Stem Cell Unit and
Anatomy Department in general, and under the umbrella of
a single goal of providing high-quality technical and
research services.
Dr. Muhammad Atteya
Ali Hasan, M.D., Supervisor of the Histology Service
Laboratory and Administrator of the ScanScope system for
virtual microscopy and image analysis. Assistant
Professor of Histology in the Anatomy Department and a
member of the Stem Cell Unit. Specially talented in
photomicrography, image analysis and statistics.
Contact information:
Tel office: +966 14679554 Fax: +966 14671300 Email:
mhasan1@ksu.edu.sa
Website: http://www.stemcell.org.sa
Ali Al-Roalle, M.Sc., Histology specialist and a
member of the Stem Cell Unit. Performs various
histological techniques, including tissue processing,
sectioning and staining. Performs the training on
paraffin and frozen sectioning and staining. Specially
talented in dissection, sample acquisition and
development and validation of tissue processing and
various staining protocols.
Rokhsana Aziz, B.Sc.,Technician. Performs various
histological techniques, including tissue processing,
sectioning and staining.
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